Jason H. Brickner Cell biology of transcription

Research Interests

The genome is spatially organized within the nucleus. The position of individual genes is dynamically regulated and impacts their expression.

The Brickner lab studies the molecular mechanisms that control gene positioning in budding yeast and human cells.  Current work in the lab is focused on three areas: 1) defining the molecular mechanisms by which transcription factors target genes to different subnuclear environments, 2) how gene positioning is integrated with interchromosomal clustering to influence the spatial organization of the genome as a whole and 3) exploring how gene positioning impacts chromatin structure, transcription and epigenetic regulation.  We employ molecular genetics, biochemistry, live cell microscopy and genomics to address these questions.

Selected Publications

ChEC-seq2: an improved Chromatin Endogenous Cleavage sequencing method and bioinformatic analysis pipeline for mapping in vivo protein-DNA interactions (2024). Van Belzen, J., C. Duan, D.G. Brickner & J.H. Brickner. NAR Genomics & Bioinformatics (6), Issue 1: lqae012

Mitotically heritable, RNA polymerase II-independent H3K4 dimethylation stimulates INO1 transcriptional memory. Sump B, Brickner DG, D'Urso A, Kim SH, and Brickner JH. eLife. 2022 May 17;11:e77646.

Random sub-diffusion and capture of genes by the nuclear pore reduces dynamics and coordinates interchromosomal movement. Sumner MC, Torrisi SB, Brickner DG, and Brickner JH. eLife. 2021 May 18;10:e66238.

The Role of Transcription Factors and Nuclear Pore Proteins in Controlling the Spatial Organization of the Yeast Genome. Brickner DG, Randise-Hinchliff C, Corbin ML, Liang JM, Kim S, Sump B, D'Urso A, Kim SH, Satomura A, Schmit H, Coukos R, Hwang S, Watson R, and Brickner JH. Developmental Cell. 2019 June 17;49(6):P936-947.E4.

Genetic and Epigenetic Strategies Potentiate Gal4 Activation to Enhance Fitness in Recently Diverged Yeast Species. Sood V and Brickner JH. Current Biology. 2017 December 4;27(23):3591-3602.e3.

Set1/COMPASS and Mediator are repurposed to promote epigenetic transcriptional memory. D'Urso A, Takahashi Y-H, Xiong B, Marone J, Coukos R, Randise-Hinchliff C, Wang J-P, Shilatifard A, and Brickner JH. eLife. 2016 June 23;5:e16691.

Strategies to regulate transcription factor–mediated gene positioning and interchromosomal clustering at the nuclear periphery. Randise-Hinchliff C, Coukos R, Sood V, Sumner MC, Zdraljevic S, Meldi Sholl L, Brickner DG, Ahmed S, Watchmaker L, and Brickner JH. Journal of Cell Biology. 2016 March 14;212(6):633-646.

View all publications by Jason H. Brickner listed in the National Library of Medicine (PubMed). Current and former IBiS students in blue.